Chancellor Rishi Sunak is being urged to "stop endless rises in upfront costs that strangle businesses" ahead of next week's Spring Budget.
The British Chambers of Commerce (BCC) wants the Chancellor to deliver a Budget to help businesses and entrepreneurs thrive after 1 January 2021.
From that point, the transitional period that currently sees EU rules remain in place in the UK will come to an end.
The BCC warned that businesses face increasing costs to move goods across borders, hire employees and comply with tax rules and regulations.
The Government should also avoid introducing new bureaucracy and compliance requirements when the Budget is delivered on 11 March.
Adam Marshall, director-general at the BCC, said:
"The Chancellor has a huge opportunity to go ‘all in' and take big steps to improve conditions for UK business and enterprise.
"An opportunity to stop the endless rises in upfront costs, to back innovators and entrepreneurs, and to send a signal of support to British business through this period of political change."
We will provide coverage of Spring Budget 2020.